Journal: Cerebellum (London, England)

Article Title: Longitudinal Study and Characterization of Gait Impairment in a Mouse Model of SCA1

doi: 10.1007/s12311-025-01910-2

Figure Lengend Snippet: Phenotypes of wild type C57BL/6J and heterozygous SCA1 154Q/2Q mice tracked through disease stages. During the pre-symptomatic stage of SCA1, heterozygous male and female mice present a phenotype similar to their wild type littermates. At the onset of SCA1, around 20 weeks old, mice begin to show early signs of hindlimb clasping. When heterozygous mice reach 30 weeks old, moderate disease symptoms show with mice expressing kyphosis, muscle wasting, a shortening of body length, and a lowering of stature. Nearing the end of the disease, at 40 weeks old, mice show severe disease phenotypes, including severe decrease in overall gate speed, muscle tone, and body weight along with kyphosis, hindlimb clasping, and hindquarter dragging

Article Snippet: Initially, two SCA1 154Q/2Q transgenic male mice (stock number #005601, B6.129S7-Atxn1 tm1Hzo /J), originally purchased from Jackson laboratories (Bar Harbor, ME), were bred with WT C57BL/6J female mice; breeding pairs and colonies were housed in the Animal Resource Facility at AMC.

Techniques: Expressing

Journal: Cerebellum (London, England)

Article Title: Longitudinal Study and Characterization of Gait Impairment in a Mouse Model of SCA1

doi: 10.1007/s12311-025-01910-2

Figure Lengend Snippet: a The figure represents mean hindlimb clasping behavior scores for WT and SCA1 154Q/2Q from 8–42 weeks. b A Kruskal-Wallis test was used to compare differences between all groups at ages 8 weeks, 20 weeks, 30 weeks, and 40 weeks. Asterisks indicate significance: (*) denotes p < 0.05, (**) p < 0.01, and (***) denotes p < 0.001. Sample size for each timepoint for WT male, SCA1 154Q/2Q male, WT female, and SCA1 154Q/2Q female are as follows: 10 weeks ( n = 6, 6, 5, 5), 20 weeks ( n = 12, 4, 6, 4), 30 weeks ( n = 10, 11, 9, 6), and 40 weeks ( n = 8, 13, 11, 7)

Article Snippet: Initially, two SCA1 154Q/2Q transgenic male mice (stock number #005601, B6.129S7-Atxn1 tm1Hzo /J), originally purchased from Jackson laboratories (Bar Harbor, ME), were bred with WT C57BL/6J female mice; breeding pairs and colonies were housed in the Animal Resource Facility at AMC.

Techniques:

Journal: Cerebellum (London, England)

Article Title: Longitudinal Study and Characterization of Gait Impairment in a Mouse Model of SCA1

doi: 10.1007/s12311-025-01910-2

Figure Lengend Snippet: Gait speed of wild type C57BL/6J and heterozygous SCA1 154Q/2Q male and female mice on incline, flat, and decline angles. The side Y-axis shows an enlarged view of value range (0–25 cm/s). The X-axis ranges from the ages of weeks 7–42. A mixed effects analysis with repeated measures was conducted between the SCA1 154Q/2Q and WT groups. Asterisks indicate significance: (*) denotes p < 0.05

Article Snippet: Initially, two SCA1 154Q/2Q transgenic male mice (stock number #005601, B6.129S7-Atxn1 tm1Hzo /J), originally purchased from Jackson laboratories (Bar Harbor, ME), were bred with WT C57BL/6J female mice; breeding pairs and colonies were housed in the Animal Resource Facility at AMC.

Techniques:

Journal: Cerebellum (London, England)

Article Title: Longitudinal Study and Characterization of Gait Impairment in a Mouse Model of SCA1

doi: 10.1007/s12311-025-01910-2

Figure Lengend Snippet: Stride length (SL) of wild type C57BL/6J and heterozygous SCA1 154Q/2Q male and female mice on incline, flat, and decline angles. The side Y-axis shows an enlarged view of value range (0–8 cm). The X-axis ranges from the ages of weeks 7–42. A mixed effects analysis with repeated measures was conducted between the SCA1 154Q/2Q and WT groups. Asterisks indicate significance: (*) denotes p < 0.05

Article Snippet: Initially, two SCA1 154Q/2Q transgenic male mice (stock number #005601, B6.129S7-Atxn1 tm1Hzo /J), originally purchased from Jackson laboratories (Bar Harbor, ME), were bred with WT C57BL/6J female mice; breeding pairs and colonies were housed in the Animal Resource Facility at AMC.

Techniques:

Journal: Cerebellum (London, England)

Article Title: Longitudinal Study and Characterization of Gait Impairment in a Mouse Model of SCA1

doi: 10.1007/s12311-025-01910-2

Figure Lengend Snippet: MAX dA/dt (cm 2 /s) of wild type C57BL/6J and heterozygous SCA1 154Q/2Q mice. The side Y-axis shows an enlarged view of value range (0-120 cm 2 /s). The X-axis shows the comparison between WT and SCA1 154Q/2Q LF, RF, LH, and RH limbs. A two-way ANOVA was conducted between the SCA1 154Q/2Q and WT groups. Asterisks indicate significance: (*) denotes p < 0.05, (**) denotes p < 0.01, (***) denotes p < 0.001, and (****) denotes p < 0.0001

Article Snippet: Initially, two SCA1 154Q/2Q transgenic male mice (stock number #005601, B6.129S7-Atxn1 tm1Hzo /J), originally purchased from Jackson laboratories (Bar Harbor, ME), were bred with WT C57BL/6J female mice; breeding pairs and colonies were housed in the Animal Resource Facility at AMC.

Techniques: Comparison

Journal: Cerebellum (London, England)

Article Title: Longitudinal Study and Characterization of Gait Impairment in a Mouse Model of SCA1

doi: 10.1007/s12311-025-01910-2

Figure Lengend Snippet: Brake time(s) of wild type C57BL/6J and heterozygous SCA1 154Q/2Q mice. The side Y-axis shows an enlarged view of value range (0.00–025 s). The X-axis shows the comparison between WT and SCA1 154Q/2Q LF, RF, LH, and RH limbs. A two-way ANOVA was conducted between the SCA1 154Q/2Q and WT groups. Asterisks indicate significance: (*) denotes p < 0.05, (**) denotes p < 0.01, and (***) denotes p < 0.001

Article Snippet: Initially, two SCA1 154Q/2Q transgenic male mice (stock number #005601, B6.129S7-Atxn1 tm1Hzo /J), originally purchased from Jackson laboratories (Bar Harbor, ME), were bred with WT C57BL/6J female mice; breeding pairs and colonies were housed in the Animal Resource Facility at AMC.

Techniques: Comparison

Journal: Cerebellum (London, England)

Article Title: Longitudinal Study and Characterization of Gait Impairment in a Mouse Model of SCA1

doi: 10.1007/s12311-025-01910-2

Figure Lengend Snippet: Propulsion time(s) of wild type C57BL/6J and heterozygous SCA1 154Q/2Q mice. The side Y-axis shows an enlarged view of value range (0.0–0.5 s). The X-axis shows the comparison between WT and SCA1 154Q/2Q LF, RF, LH, and RH limbs. A two-way ANOVA was conducted between the SCA1 154Q/2Q and WT groups. Asterisks indicate significance: (*) denotes p < 0.05, and (**) denotes p < 0.01

Article Snippet: Initially, two SCA1 154Q/2Q transgenic male mice (stock number #005601, B6.129S7-Atxn1 tm1Hzo /J), originally purchased from Jackson laboratories (Bar Harbor, ME), were bred with WT C57BL/6J female mice; breeding pairs and colonies were housed in the Animal Resource Facility at AMC.

Techniques: Comparison

Journal: Cerebellum (London, England)

Article Title: Longitudinal Study and Characterization of Gait Impairment in a Mouse Model of SCA1

doi: 10.1007/s12311-025-01910-2

Figure Lengend Snippet: Stance time(s) of wild type C57BL/6J and heterozygous SCA1 154Q/2Q mice. The side Y-axis shows an enlarged view of value range (0.0–0.5 s). The X-axis shows the comparison between WT and SCA1 154Q/2Q LF, RF, LH, and RH limbs. A two-way ANOVA was conducted between the SCA1 154Q/2Q and WT groups. Asterisks indicate significance: (*) denotes p < 0.05, and (**) denotes p < 0.01

Article Snippet: Initially, two SCA1 154Q/2Q transgenic male mice (stock number #005601, B6.129S7-Atxn1 tm1Hzo /J), originally purchased from Jackson laboratories (Bar Harbor, ME), were bred with WT C57BL/6J female mice; breeding pairs and colonies were housed in the Animal Resource Facility at AMC.

Techniques: Comparison

Journal: Cerebellum (London, England)

Article Title: Longitudinal Study and Characterization of Gait Impairment in a Mouse Model of SCA1

doi: 10.1007/s12311-025-01910-2

Figure Lengend Snippet: Perfused brain weights of wild type C57BL/6J and heterozygous SCA1 154Q/2Q mice. At the end of the test period of 42 weeks, mice were perfused trans-cardiac approach with heparin, followed by 4% PFA in saline. A two-way ANOVA showed that brains of WT male and female mice weighed more than their SCA1 154Q/2Q littermates, (****) denotes p < 0.0001. The sample size for each group is shown at the bottom of each bar

Article Snippet: Initially, two SCA1 154Q/2Q transgenic male mice (stock number #005601, B6.129S7-Atxn1 tm1Hzo /J), originally purchased from Jackson laboratories (Bar Harbor, ME), were bred with WT C57BL/6J female mice; breeding pairs and colonies were housed in the Animal Resource Facility at AMC.

Techniques: Saline

Journal: Cerebellum (London, England)

Article Title: Longitudinal Study and Characterization of Gait Impairment in a Mouse Model of SCA1

doi: 10.1007/s12311-025-01910-2

Figure Lengend Snippet: Summary of gait and locomotion metrics from WT and SCA1 male and female mice at four specific ages in our longitudinal study design. An arrow indicates significance, and the direction denotes increase or decrease; ns denotes ‘no significant difference’

Article Snippet: Initially, two SCA1 154Q/2Q transgenic male mice (stock number #005601, B6.129S7-Atxn1 tm1Hzo /J), originally purchased from Jackson laboratories (Bar Harbor, ME), were bred with WT C57BL/6J female mice; breeding pairs and colonies were housed in the Animal Resource Facility at AMC.

Techniques:

Journal: bioRxiv

Article Title: Type 2 Deiodinase in Cancer-Associated Fibroblasts is required to sustain growth of poorly and undifferentiated thyroid cancer

doi: 10.1101/2025.07.10.664131

Figure Lengend Snippet: (A) Thyroid cancer mouse model: TPO-Cre/LSL-BrafV600E/p53 lox-lox /eYFP. (B) Representative images of thyroid tumors in the ATC mouse model at 6-8 and 18-21 weeks of age (Scale bar, 1cm). (C) Images of H&E staining of thyroid tumor section from ATC mouse model at 6-8 and 18-21 weeks (Scale bar, 100 μ m). (D) Tumor thyroid weights from ATC mice at 6-8 and 18-21 weeks. Each dot represents an individual animal (6-8 weeks, n=6; 18-21 weeks, n=11). (E) D2 and D3 mRNA expression levels in thyroids from ATC mice at different ages compared to normal thyroid tissue. Each point represents the mean of pooled samples from several animals (2-3 weeks, n= 3; 6-8 weeks, n=6; 13-15 weeks, n=7; and 18-21 weeks, n= 11). (F) Representative blow cytometry dot plot showing YFP + (green) and YFP − (red) cells. (G) Percentage of epithelial (YFP + ) and mesenchymal (YFP − ) cells from pooled thyroid tumors of ATC mice at 20 weeks, analyzed by FACS. Each point represents an individual experiment (pooled tumors from n=3 mice). (H) D2 mRNA expression in YFP + and YFP − cells sorted from ATC thyroid tumors at 20 weeks. Each point represents a technical replicate from pooled tumors (n=3 mice). (I) In silico analysis of scRNA-seq data extracted from Lu et al. . (J) Representative blow cytometry dot plot showing YFP + (green) and SCA1 + (violet) cells. (K) Percentage of epithelial (YFP + ) and CAF (SCA + ) cells in pooled thyroid tumors from 20-week-old ATC mice, analyzed by FACS. Each point represents an individual experiment (pooled tumors from n=3 mice). (L) D2 mRNA expression in thyroid tumors at 8 and 20 weeks and in YFP + and SCA + and YFP − /SCA1 − cells sorted at 20 weeks. (M-N) Col1a1 (M) and IL6 (N) mRNA expression in thyroid tumors at 8 and 20 weeks and in YFP + and SCA + cells sorted from tumors at 20 weeks. Each point represents an individual experiment. Data are expressed as mean ± S.E.M. * P < 0.05, ** P < 0.01, ***P < 0.0001 using t tests in D, G, H and K or ANOVA in E, L, M and N.

Article Snippet: The blow through fraction was collected and incubated with an anti-Sca1-microbeads antibody (Miltenyi Biotec #130–106– 641).

Techniques: Staining, Expressing, Cytometry, In Silico